Stage 13

 

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 13 - 3D model by SangerLab (@SangerLab) [3ab701f]

Anolis sagrei stage 13- embryo - 3D model by SangerLab (@SangerLab) [e410b39]

Pinching at distal tips has formed into claws.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 13 - brain - 3D model by SangerLab (@SangerLab) [6e979ec]

No obvious changes in neural morphology.

Incubated at 27°C Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size - Anolis sagrei stage 13 - skull - 3D model by SangerLab (@SangerLab) [d89d262]

Jugal: increased ossification
Parietal: Ossification of the parietal is first seen at stage 13, as two slender angled slivers of bone. This will eventually expand to cover the central and upper back part of each side of the skull.