Stage 12
Cerebellum and optic tectum have increased in size again compared to previous stage.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 5: brain - 3D model by SangerLab (@SangerLab) [15294c8]
Developing cephalic bulges are distinct enough to distinguish into the cerebrum, optic tectum, and hindbrain.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 6 :brain - 3D model by SangerLab (@SangerLab) [e3b6d60]
The dorsal bulge that makes up the optic tectum is more exaggerated than in previous stage.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 7: brain - 3D model by SangerLab (@SangerLab) [c36421e]
The cerebellum begins to form a more distinctive bulge caudo-ventrally.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 8: brain - 3D model by SangerLab (@SangerLab) [5095378]
Continued growth of the cerebrum and cerebellum.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 9:brain - 3D model by SangerLab (@SangerLab) [085c4e7]
The beginning of the olfactory bulb starts forming at the dorso-frontal position. Continued growth of the cerebrum and cerebellum.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 10:brain - 3D model by SangerLab (@SangerLab) [5d359d7]
Olfactory bulb has grown. The areas around the cerebrum and optic tectum are noticeably larger. Cerebellum and hindbrain have grown.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 11 : brain - 3D model by SangerLab (@SangerLab) [8759c4f]
Regions of the brain, especially around the cerebellum and optic tectum, have increased in size compared to previous stage.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 12: brain - 3D model by SangerLab (@SangerLab) [4aa7a1a]
Cerebellum and optic tectum have increased in size again compared to previous stage.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 13 : brain - 3D model by SangerLab (@SangerLab) [5af1a87]
Cerebellum and optic tectum are less distinct.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 14: brain - 3D model by SangerLab (@SangerLab) [06321d4]
Optic tectum and cerebellum are less distinct.
Anolis sagrei stage 15: brain - 3D model by SangerLab (@SangerLab) [aec1b97]
Olfactory bulb has frown longer than in previous stage. Hindbrain has increased in size. Optic tectum and cerebellum are less distinct.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei brain: stage 16 - 3D model by SangerLab (@SangerLab) [6ff2429]
Optic tectum and cerebellum are less distinct.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 17 : brain - 3D model by SangerLab (@SangerLab) [a1acc0b]
No change from previous stage.
Anolis sagrei stage 18 :brain - 3D model by SangerLab (@SangerLab) [e667363]
No change from previous stage.
Anolis sagrei stage 19: brain - 3D model by SangerLab (@SangerLab) [00a6725]
No change from previous stage.