Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - stage 5 - 3D model by SangerLab (@SangerLab) [2b12c3d]
Both front and hind limb buds wider than they are long. Hindlimb buds are slightly bigger than forelimb.
Anolis sagrei embryo - Stage 6 - Anolis sagrei embryo - Stage 6 - 3D model by SangerLab (@SangerLab) [2a1b5e6]
Hindlimb more distinct than forelimb. Distal limb buds are paddle shaped.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - stage 7 - 3D model by SangerLab (@SangerLab) [0aeb1ed]
Paddles wider than in previous stage. Medial digit condensation beginning to be visible.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - stage 8 - 3D model by SangerLab (@SangerLab) [d36aaf2]
Condensation of digit cartilages are visible with slight thinning of internal webbing but no regression.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - stage 9 - 3D model by SangerLab (@SangerLab) [6e56628]
Distal tips of digits freed of internal webbing. 4th digit noticeably larger.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - Stage 10 - 3D model by SangerLab (@SangerLab) [8d0ab79]
Digital webbing receeded to half its length on digits. Slightly elongated medial digits.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei stage 11 - full body - 3D model by SangerLab (@SangerLab) [8c63b31]
Digital webbing has fully regressed. Pinching at distal tips eventually will form claws.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size - Anolis sagrei stage 12 - fullbody - 3D model by SangerLab (@SangerLab) [ccba0bd]
Further pinching at distal tips.
Anolis sagrei fullbody - stage 13 - 3D model by SangerLab (@SangerLab) [e410b39]
Pinching at distal tips has formed into claws.
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolios sagrei embryo - stage 14 - 3D model by SangerLab (@SangerLab) [3b5c842]
Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size - Anolis sagrei stage 15 - head and torso - 3D model by SangerLab (@SangerLab) [c24f1e2]
